Genome database UCSC - Homo sapien - hg38 - Genome Human T2T Genome (GCA_009914755.4) UCSC - Homo sapien - hg19 - Genome UCSC - Anolis carolinensis - anoCar2 - Genome UCSC - Anopheles gambiae v. MOZ2 - anoGam1 - Genome UCSC - Apis mellifera - apiMel2 - Genome UCSC - Aplysia californica - aplCal1 - Genome UCSC - Apteryx australis - aptMan1 - Genome UCSC - Aquila chrysaetos canadensis - aquChr2 - Genome UCSC - Balaenoptera acutorostrata scammoni - balAcu1 - Genome UCSC - Bison bison - bisBis1 - Genome UCSC - Bos taurus - bosTau8 - Genome UCSC - Branchiostoma floridae - braFlo1 - Genome UCSC - Caenorhabditis japonica - caeJap1 - Genome UCSC - Caenorhabditis brenneri (PB2801) - caePb2 - Genome UCSC - Caenorhabditis remanei - caeRem3 - Genome UCSC - Callithrix jacchus - calJac1 - Genome UCSC - Callithrix jacchus - calJac3 - Genome UCSC - Callorhinchus milii - calMil1 - Genome UCSC - Canis familiaris - canFam1 - Genome UCSC - Canis familiaris - canFam3 - Genome UCSC - Cavia porcellus - cavPor3 - Genome UCSC - Caenorhabditis briggsae - cb3 - Genome UCSC - Caenorhabditis elegans - ce10 - Genome UCSC - Caenorhabditis elegans - ce11 - Genome UCSC - Caenorhabditis elegans - ce6 - Genome UCSC - Ceratotherium simum - cerSim1 - Genome UCSC - Chlorocebus sabaeus - chlSab2 - Genome UCSC - Choloepus hoffmanni - choHof1 - Genome Painted turtle genomic DNA from UCSC chrPic1 UCSC - Ciona intestinalis (sea squirt) - ci3 - Genome UCSC - Cricetulus griseus - criGri1 - Genome UCSC - Cricetulus griseus - criGriChoV1 - Genome UCSC - Danio rerio - danRer11 - Genome UCSC - Dasypus novemcinctus - dasNov3 - Genome UCSC - Dipodomys ordii - dipOrd1 - Genome UCSC - Drosophila melanogaster - dm6 - Genome UCSC - Drosophila pseudoobscura - dp3 - Genome UCSC - Drosophila ananassae - droAna2 - Genome UCSC - Drosophila erecta - droEre1 - Genome UCSC - Drosophila grimshawi - droGri1 - Genome UCSC - Drosophila mojavensis - droMoj2 - Genome UCSC - Drosophila persimilis - droPer1 - Genome UCSC - Drosophila sechellia - droSec1 - Genome UCSC - Drosophila simulans - droSim1 - Genome UCSC - Drosophila virilis - droVir2 - Genome UCSC - Drosophila yakuba - droYak2 - Genome UCSC - Filoviridae ebolavirus - eboVir3 - Genome UCSC - Echinops telfairi - echTel2 - Genome UCSC - Equus caballus - equCab2 - Genome UCSC - Erinaceus europaeus - eriEur2 - Genome UCSC - Felis catus - felCat8 - Genome UCSC - Takifugu rubripes v5.0 - fr3 - Genome UCSC - Gadus morhua - gadMor1 - Genome UCSC - Gallus gallus - galGal5 - Genome UCSC - Galeopterus variegatus - balAcu1 - Genome UCSC - Gasterosteus aculeatus - gasAcu1 - Genome UCSC - Geospiza fortis - geoFor1 - Genome UCSC - Gorilla gorilla gorilla - gorGor5 - Genome UCSC - Heterocephalus glaber - hetGla2 - Genome UCSC - Homo sapien - hg16 - Genome UCSC - Homo sapien - hg18 - Genome UCSC - Latimeria chalumnae - latCha1 - Genome UCSC - Loxodonta africana - loxAfr3 - Genome UCSC - Notamacropus eugenii - macEug2 - Genome UCSC - Macaca fascicularis - macFas5 - Genome UCSC - Manis pentadactyla - manPen1 - Genome UCSC - Meleagris gallopavo - melGal5 - Genome UCSC - Melopsittacus undulatus - melUnd1 - Genome UCSC - Microcebus murinus - micMur2 - Genome UCSC - Mus musculus - mm39 - Genome UCSC - Mus musculus - mm10 - Genome UCSC - Monodelphis domestica - monDom5 - Genome UCSC - Mustela putorius furo - musFur1 - Genome UCSC - Myotis lucifugus - myoLuc2 - Genome UCSC - Nanorana parkeri - nanPar1 - Genome UCSC - Nasalis larvatus - nasLar1 - Genome UCSC - Nomascus leucogenys - nomLeu3 - Genome UCSC - Ochotona princeps - ochPri3 - Genome UCSC - Oreochromis niloticus - oreNil2 - Genome UCSC - Ornithorhynchus anatinus - ornAna2 - Genome UCSC - Oryctolagus cuniculus - oryCun2 - Genome UCSC - Oryzias latipes - oryLat2 - Genome UCSC - Otolemur garnettii - otoGar3 - Genome UCSC - Ovis aries - oviAri3 - Genome UCSC - Pan paniscus - panPan2 - Genome UCSC - Pan troglodytes - panTro5 - Genome UCSC - Papio anubis - papAnu2 - Genome UCSC - Papio hamadryas - papHam1 - Genome UCSC - Petromyzon marinus - petMar2 - Genome UCSC - Pongo abelii - ponAbe2 - Genome UCSC - Pristionchus pacificus (5.0) - priPac1 - Genome UCSC - Procavia capensis - proCap1 - Genome UCSC - Pteropus vampyrus - pteVam1 - Genome UCSC - Rhesus macaque - rheMac8 - Genome UCSC - Rhinopithecus roxellana - rhiRox1 - Genome UCSC - Rattus norvegicus - rn6 - Genome UCSC - Saccharomyces cerevisiae - sacCer3 - Genome UCSC - Saimiri boliviensis - saiBol1 - Genome UCSC - Sarcophilus harrisii - sarHar1 - Genome UCSC - Sorex araneus - sorAra2 - Genome UCSC - Spermophilus tridecemlineatus - speTri2 - Genome UCSC - Strongylocentrotus purpuratus - strPur2 - Genome UCSC - Sus scrofa - susScr11 - Genome UCSC - Taeniopygia guttata - taeGut2 - Genome UCSC - Tarsius syrichta - tarSyr2 - Genome UCSC - Tetraodon nigroviridis - tetNig2 - Genome UCSC - Trichechus manatus latirostris - triMan1 - Genome UCSC - Tupaia belangeri - tupBel1 - Genome UCSC - Tursiops truncatus - turTru2 - Genome UCSC - Vicugna pacos - vicPac2 - Genome UCSC - Xenopus laevis - xenLae2 - Genome UCSC - Xenopus tropicalis - xenTro9 - Genome Human GRCh37 RNA Human GRCh38 RNA Mouse GRCm38 RNA Potato Genome Sequence (DM_v4.04_pseudomolecules) Prunus persica v2.0.a1 scaffolds Pseudomonas Genomic DNA (pgd_r_16_2) Phaseolus vulgaris v2.1 genome Rice Genome release 7 Arabidopsis thaliana Genome (TAIR10) UNITE public 22.08.2016 Zea mays (AGPv4) Drosophila melanogaster genome (fruit fly assembly Release 6 plus ISO1 MT)- Rattus norvegicus RNA (Norway rat assembly Rnor_6.0) Danio rerio genome (zebrafish assembly GRCz11) Danio rerio RNA (zebrafish assembly GRCz11) Apis mellifera genome (honey bee assembly Amel_4.5) Apis mellifera RNA (honey bee assembly Amel_4.5) Caenorhabditis elegans (assembly WBcel235) RNA Sus scrofa (pig) representative genome Sscrofa11.1 Sus scrofa (pig) RNA Sscrofa11.1 C.chinense(assembly Chinense.v1.2.Total) Capsicum_annuum(assembly Capsicum_annuum_UCD10X_v.1.0) Capsicum_annuum(assembly Annuum.v1.6.Total) C. baccatum(assembly Baccatum.v1.2.Total) Bubalus bubalis (water buffalo)-All 4 genomes for species Fragaria_x_ananassa_Genome_v1.0_FAN_r1.1_scaffolds.fasta Sorghum bicolor (assembly Sorghum_bicolor_NCBIv3) Bovine coronavirus(viruses) "Myxococcus xanthus DK 1622, complete genome" Haloferax volcanii(Representative genome Haloferax volcanii DS2) Chlamydia trachomatis(Reference genome Chlamydia trachomatis 434/Bu) Neisseria gonorrhoeae(Reference genome Neisseria gonorrhoeae FA 1090) Corynebacterium kutscheri Streptococcus pneumoniae(Reference genome: Streptococcus pneumoniae R6) Mycoplasma pulmonis(Representative genome Mycoplasma pulmonis UAB CTIP) Bos_taurus_UMD_3.1.1 assembly for Bos taurus Salmonella enterica subsp. enterica serovar Typhimurium str. LT2(genome) Salmonella enterica subsp. enterica serovar Enteritidis str. P125109 (genome) Spirodela polyrhiza (assembly ASM198140v1) Bacillus cereus(Reference genome Bacillus cereus ATCC 14579) Pelodiscus sinensis (assembly PelSin_1.0) Physcomitrella patens (assembly Phypa V3) Trichomonas vaginalis G3 (assembly ASM282v1) Solanum lycopersicum (assembly SL3.0) Solanum tuberosum (assembly SolTub_3.0) Castor canadensis (assembly C.can genome v1.0) Vaccinia virus WR (complete genome) African swine fever virus Clostridium perfringens (Representative genome Clostridium perfringens ATCC 13124) Stenotrophomonas maltophilia K279a (complete genome) longan (Dimocarpus longan Lour.) Oryza sativa Japonica (IRGSP-1.0) Oryza sativa Japonica (cDNA) Leishmania KDNA Oilseed rape (Brassica napus L.) Alphavirus (nucleotide) BosGru_v2.0_genomic Araport11_genes (cdna) Medicago truncatula (assembly MedtrA17_4.0) Oreochromis niloticus (Refseq mRNA) Manihot esculenta (assembly Manihot esculenta v6) Moniliophthora perniciosa FA553 (assembly ASM18302v1) Moniliophthora roreri (assembly ASM146670v1) Macaca_fascicularis (RNA) Hepacivirus (HCV data set) Triticum aestivum (bread wheat assembly iwgsc_refseqv1.0) Aspergillus niger (16 genomes) Pekin duck, GCF_015476345.1) Glycine max (soybean), GCF_000004515.6 USDA_OmykA_1.1 (Oncorhynchus mykiss) Omyk_1.0 (Oncorhynchus mykiss) USDA_OmykWR_1.0 (Oncorhynchus mykiss) USDA_OmykKC_1.0 (Oncorhynchus mykiss) Otsh_v2.0 (Oncorhynchus tshawytscha) Otsh_v1.0 (Oncorhynchus tshawytscha) Okis_V2 (Oncorhynchus kisutch) Oner_1.1 (Oncorhynchus nerka) ETRf_v1 (Entosphenus tridentatus) MU_Atrans_1.0 (Acipenser transmontanus) Anser cygnoides domesticus (swan goose) Tremarctos ornatus (spectacled bear) dog genome, Oct. 2020 (Dog10K_Boxer_Tasha/canFam6) Spinacia oleracea (spinach) GCA_020520425.1 2021-10-20 Cuscuta campestris (v0.32) genome Taihu goose T2T genome (GCF_040182565.1) Gallus gallus (chicken) bGalGal1, GRCg7b, GCF_016699485.2

Targets in BED format (<= 100, example, clear) Maximum 100 lines, each region ≤ 10M bp

Chromosome name examples chrM 1000 1001 chrM 1000 2000 chrM 3000 3100

Left flank (bp) Add to 5' position

Right flank (bp) Add to 3' position

Reverse sequence Output the reverse sequence (5' → 3' becomes 3' → 5')

Complement sequence Output the complement sequence (use with Reverse for reverse-complement)

Run