MFEprimer-4.4.0: PCR Primer Design and Quality Control Tools

Genome database

Targets in BED format (<= 100, example, clear) Maximum 100 lines, each region ≤ 10M bp

Chromosome name examples

chrM    1000 1001
chrM    1000 2000
chrM    3000 3100

Left flank (bp) Add to 5' position

Right flank (bp) Add to 3' position

Reverse sequence Output the reverse sequence (5' → 3' becomes 3' → 5')

Complement sequence Output the complement sequence (use with Reverse for reverse-complement)

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Retrieve subsequence from FASTA